Research Journal of Pharmacy and Technology

Radha Krishna Kollipara ¹, Vyshnavi Tallapaneni ¹, Bharat Kumar Reddy Sanapalli ¹, G. Vinoth Kumar ¹, Veera Venkata Satyanarayana Reddy Karri ¹

Affiliations
1 Department of Pharmaceutics, JSS College of Pharmacy, Ootacamund, JSS Academy of Higher Education and Research, Mysuru, Karnataka, IN

Abstract

Curcumin has an excellent safety profile having various pleiotropic actions, including anti-inflammatory, antioxidant, antitumoral, anticancer, and antimicrobial activities, with the potential for neuroprotective activity but the poor oral bioavailability limits its use as oral dosage form. The purpose of this study was to formulate and evaluate the transdermal drug delivery system of Ethosomes containing curcumin, a potent anti-cancer drug. Study on various formulation excipients revealed that all the excipients shown some variation which affected the vesicle size, zeta potential, PDI and entrapment efficiency. Batch prepared with 10 % of soya lecithin, 4.5 % of ethanol and 10% of cholesterol showed maximum entrapment efficiency of 81.2±3.12.In in vitro drug permeation studies using dialysis bag, the samples withdrawn in equal time intervals shown more drug release from the dialysis bag in comparison with that of inhouse paclitaxel gel which conform that the curcumin loaded ethosomal gel is a better formulation for the further studies. In ex vivo, permeation study using pork ear skin, curcumin loaded ethosomal gel showed the greater drug deposition on the skin > 60 % in 12 hrs for the better curability for the melanoma. Whereas inhouse paclitaxel gel shown < 60 % of drug deposition in 12 h. The comparison of both ethosomal gel and inhouse paclitaxel gel reveals that ethosomal gel shown maximum release within 12 hrs in both in vitro and ex vivo studies, which can be taken for the further in vitro cell line and in vivo study.

Keywords

Curcumin, Anticancer, Ethosomes, Melanoma, PDI.

Full Text

     

C. Kalaivani ¹, Gowthamarajan Kuppusamy ¹, Saikamal ¹, Veera Venkata Satyanarayana Reddy Karri ¹

Affiliations
1 Department of Pharmaceutics, JSS College of Pharmacy, Ooty, IN

Abstract

Scaffolds are the adaptable tools for the treatment of diabetic wounds, In this study, Simvastatin was loaded in polycaprolactone-collagen scaffolds for the treatment of diabetic foot ulcer using the freeze-drying technique. Eventually scaffolds characterization were observed in terms of Scanning Electron Microscopy, Differential scanning calorimetry (DSC), Porosity, Water absorption test, Matrix degradation, In vitro drug release studies, cell proliferation assay, cytotoxicity assay. Scaffolds prepared with polycaprolactone and collagen-polymer showed the homogeneous distribution with high porosity, extended biodegradation rate, high water uptake. All the scaffold formulation showed the sustained drug release and then a plateau. The cross-Linked scaffold has significant slower release in comparison to non-crosslinked scaffold, this is because of cross-linking of the scaffolds where the chemical and mechanical bonding is high compared to non-crosslinked scaffold, Non-crosslinked, showed 85.34% of drug release by this it can be concluded that non cross-linked scaffolds showed the prolonged release, In the cell proliferation assay after 72 h, the cell growth was found to be greater when we compared with placebo and control due to the presence of drug, which explains that the cells are in logarithmic phase. Noncross linked scaffold has shown an increase in cell growth by 28% from its initial value. In Differential Scanning Calorimetry (DSC) shows that there is no change in the peaks by that we can confirm that all the excipients are compatible to each other The obtained results imply that the investigated scaffold is a potential candidate for skin regeneration application because the present study states that simvastatin is having all the properties to treat the Diabetic Foot Ulcer(DFU) without producing the resistance.

Keywords

Simvastatin, Polycaprolactone, Collagen, Composite Scaffolds, Diabetic Wounds.

Full Text